Anti-LST1; clone 7E2

Application

Research Sub CategoryImmunological Signaling

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected LST1 in 10 µg of human brain, human lung, human liver, human kidney, human skin, human spleen, human thymus, human pancreas, human ovary, human testis, human small intestine, human parathyroid, and human adrenal gland tissue lysate.
Western Blotting Analysis: A representative lot detected LST1 in HeLa cell lysate (Schiller, C., et al. (2009). Hybridoma. 28(4):281-286).

Research CategoryInflammation & Immunology

Anti-LST1 antibody, clone 7E2 detects level of LST1 & has been published & validated for use in LST1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

LST1, also known as Leukocyte-specific transcript 1 protein, Protein B144, and encoded by the gene LST1/B144, is an interesting protein expressed in many tissues in both the developing fetus as well as the adult. Over-expression induces morphological changes in many cells types that include the production of filopodia and microspikes. LST1 is also involved in dendritic cell maturation and also has anti-proliferation activity on lymphocyte proliferation. LST1 is localized to internal and Golgi membranes and is primarily a single-pass membrane bound protein. Recent research also demonstrates that LST1 is important for the assembly of tunneling nanotubes, a recently discovered new mechanism of cell-cell communication. LST1 induces nanotube formation by recruiting the small GTPase RalA to the plasma membrane and promoting its interaction with the exocyst complex. Furthermore, LST1 recruits the actin-crosslinking protein filamin to the plasma membrane and interacts with M-Sec, myosin and myoferlin, and this suggests a molecular model for nanotube generation and LST1's essential role in their formation and maintenance.

Immunogen

His-tagged recombinant protein corresponding to human LST1.

Physical form

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected LST1 in 10 µg of human heart tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~26 kDa observed. The target is universal expressed, not leukocytes specific LST1 forms dimers through disulfide bridges.